摘要The ionic-complementary peptide EMK16-II is used to investigate the effects of hydrophobic and electrostatic interactions on the self-assembling process by atomic force microscopy and circular dichroism spectra. It is found that the increase of hydrophobicity of the peptides promotes the aggregation of fibrils in pure water. The effects of phosphate with different concentrations on electrostatic interactions are also investigated. It is found that the self-assembling process is enhanced at a low concentration of phosphate and more ordered fibrillar aggregates are formed. When the concentration of phosphate increases to a certain value (9mM), only a few fibrils are found to be formed. No fibrils but amorphous aggregates exist when the concentration further increases. A physical interpretation is presented such that one divalent anion can interact with two positively charged residual groups in different peptide molecules like a ``bridge'' which destroys the ionic-complementary feature and largely inhibits the formation of ordered fibrils.
Abstract：The ionic-complementary peptide EMK16-II is used to investigate the effects of hydrophobic and electrostatic interactions on the self-assembling process by atomic force microscopy and circular dichroism spectra. It is found that the increase of hydrophobicity of the peptides promotes the aggregation of fibrils in pure water. The effects of phosphate with different concentrations on electrostatic interactions are also investigated. It is found that the self-assembling process is enhanced at a low concentration of phosphate and more ordered fibrillar aggregates are formed. When the concentration of phosphate increases to a certain value (9mM), only a few fibrils are found to be formed. No fibrils but amorphous aggregates exist when the concentration further increases. A physical interpretation is presented such that one divalent anion can interact with two
ZOU Da-Wei;TIE Zuo-Xiu;QIN Meng;LU Chun-Mei;WANG Wei. Effect of Phosphate on the Self-Assembly of Peptide EMK16-II[J]. 中国物理快报, 2009, 26(8): 88103-088103.
ZOU Da-Wei, TIE Zuo-Xiu, QIN Meng, LU Chun-Mei, WANG Wei. Effect of Phosphate on the Self-Assembly of Peptide EMK16-II. Chin. Phys. Lett., 2009, 26(8): 88103-088103.
[1] Whitesides G M and Boncheva M 2002 Proc. Natl. Acad.Sci. U.S.A. 99 4769 [2] Fandrich M, Fletcher M A and Dobson C M 2001 Nature 410 165 [3] Booth D R, Sunde M, Bellotti V, Robinson C V, Hutchinson WL, Fraser P E, Hawkins P N, Dobson C M, Radford S E, Blake C C F andPepys M B 1997 Nature 385 787 [4] Zhang S G and Altman M 1999 React. Funct. Polym. 41 91 [5] Zhao Y, Yokoi H, Tanaka M, Kinoshita T and Tan T W 2008 Biomacromolecules 9 1511 [6] Zhang Z Q, Chen H, Bai H J and Lai L H 2007 Biophys.J. 93 1484 [7] Yang H, Fung S Y, Pritzker M and Chen P 2007 J. Am.Chem. Soc. 129 12200 [8] Holmes T C, de Lacalle S, Su X, Liu G S, Rich A and ZhangS G 2000 Proc. Natl. Acad. Sci. U.S.A. 97 6728 [9] Nowak A P, Breedveld V, Pakstis L, Ozbas B, Pine D J,Pochan D and Deming T J 2002 Nature 417 424 [10] Zhang S G, Marini D M, Hwang W and Santoso S 2002 Curr. Opin. Chem. Biol. 6 865 [11] Hartgerink J D, Beniash E and Stupp S I 2001 Science 294 1684 [12] Vauthey S, Santoso S, Gong H Y, Watson N and Zhang S G2002 Proc. Natl. Acad. Sci. U.S.A. 99 5355 [13] Whaley S R, English D S, Hu E L, Barbara P F and BelcherA M 2000 Nature 405 665 [14] Zhang S G, Holmes T, Lockshin C and Rich A 1993 Proc. Natl. Acad. Sci. U.S.A. 90 3334 [15] Zhang S G, Holmes T C, Dipersio C M, Hynes R O, Su X andRich A 1995 Biomaterials 16 1385 [16] Hong Y S, Legge R L, Zhang S and Chen P 2003 Biomacromolecules 4 1433 [17] Jun S, Hong Y, Imamura H, Ha B Y, Bechhoefer J and Chen P2004 Biophys. J. 87 1249 [18] Hong Y, Pritzker M D, Legge R L and Chen P 2005 Colloids Surf. B 46 152 [19] Hong Y S, Lau L S, Legge R L and Chen P 2004 J.Adhes. 80 913 [20] de la Paz M L, Goldie K, Zurdo J, Lacroix E, Dobson C M,Hoenger A and Serrano L 2002 Proc. Natl. Acad. Sci. U.S.A. 99 16052 [21] Chen P 2005 Colloids Surf. A 261 3 [22] Zou D W, Tie Z X, Lu C M, Qin M, Lu X M, Wang M, Wang Wand Chen P 2009 Biomacromolecules (submitted and revised) [23] Brack A and Orgel L E 1975 Nature 256 383 [24] Chamberlain A K, MacPhee C E, Zurdo J, Morozova-Roche LA, Hill H A O, Dobson C M and Davis J J 2000 Biophys. J. 79 3282 [25] Ionescu-Zanetti C, Khurana R, Gillespie J R, Petrick J S,Trabachino L C, Minert L J, Carter S A and Fink A L 1999 Proc.Natl. Acad. Sci. U.S.A. 96 13175 [26] Gale M, Pollanen M S, Markiewicz P and Goh M C 1995 Biophys. J. 68 2124 [27] Fung S Y, Keyes C, Duhamel J and Chen P 2003 Biophys. J. 85 537 [28] Yu Y, Monera O D, Hodges R S and Privalov P L 1996 J. Mol. Biol. 255 367 [29] Caplan M R, Moore P N, Zhang S G, Kamm R D andLauffenburger D A 2000 Biomacromolecules 1 627
2009, Vol. 26 
