CROSS-DISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY |
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Effects of pH on Oxaliplatin-Induced Condensation of Single DNA Molecules |
ZHANG Hong-Yan, JI Chao, LIU Yu-Ru, LI Wei, LI Hui, DOU Shuo-Xing, WANG Wei-Chi, ZHANG Ling-Yun, XIE Ping, WANG Peng-Ye** |
Key Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190
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Cite this article: |
ZHANG Hong-Yan, JI Chao, LIU Yu-Ru et al 2014 Chin. Phys. Lett. 31 028701 |
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Abstract By using magnetic tweezers, atomic force microscope and mass spectrometry, we study the effects of pH on oxaliplatin-induced DNA condensation, the DNA persistence length, the amounts of micro-loops and of oxaliplatin bound to DNA. It is found that the DNA condensation degree, the amounts of micro-loops and of oxaliplatin bound to DNA increase with the decrease in the pH value while the DNA persistence length has an opposite behavior. The observed effects may be related to the drug resistance of cancer cells.
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Received: 14 November 2013
Published: 28 February 2014
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PACS: |
87.80.Nj
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(Single-molecule techniques)
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87.64.Dz
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(Scanning tunneling and atomic force microscopy)
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82.37.Rs
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(Single molecule manipulation of proteins and other biological molecules)
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