| FUNDAMENTAL AREAS OF PHENOMENOLOGY(INCLUDING APPLICATIONS) |
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Localizing and Characterizing Colloidal Particles Scattering Using Lens-free Holographic Microscopy |
| Xia Hua†, Cheng Yang†, Ye Huang, Feng Yan, Xun Cao** |
School of Electronic Science and Engineering, Nanjing University, Nanjing 210093
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| Cite this article: |
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Xia Hua, Cheng Yang, Ye Huang et al 2019 Chin. Phys. Lett. 36 014204 |
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Abstract Lens-free holographic microscopy could achieve both improved resolution and field of view (FOV), which has huge potential applications in biomedicine, fluid mechanics and soft matter physics. Unfortunately, due to the limited sensor pixel size, target objects could not be located to a satisfactory level. Recent studies have shown that electromagnetic scattering can be fitted to digital holograms to obtain the 3D positions of isolated colloidal spheres with nanometer precision and millisecond temporal resolution. Here, we describe a lens-free holographic imaging technique that fits multi-sphere superposition scattering to digital holograms to obtain in situ particle position and model parameters: size and refractive index of colloidal spheres. We show that the proposed method can be utilized to analyze the location and character of colloidal particles under large FOV with high density.
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Received: 24 September 2018
Published: 25 December 2018
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| PACS: |
42.40.-i
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(Holography)
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42.30.Va
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(Image forming and processing)
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42.40.Lx
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(Diffraction efficiency, resolution, and other hologram characteristics)
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| Fund: Supported by the National Key R&D Program of China under Grant No 2016YFA0202102, the Fundamental Research Funds for the Central Universities under Grant No 14380078, and the Scientific Research Foundation of Graduate School of Nanjing University under Grant No 2017CL02. |
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