The Effects of Electrical Stimuli on Calcium Change and Histamine Release in Rat Basophilic Leukemia Mast Cells

doi:10.1088/0256-307X/30/6/068702

Chin. Phys. Lett.

2013, Vol. 30

Issue (6): 068702 DOI: 10.1088/0256-307X/30/6/068702

CROSS-DISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY

The Effects of Electrical Stimuli on Calcium Change and Histamine Release in Rat Basophilic Leukemia Mast Cells

ZHU Dan^**, WU Zu-Hui, CHEN Ji-Yao, ZHOU Lu-Wei^**

State Key Laboratory of Surface Physics and Department of Physics, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, Shanghai 200433

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ZHU Dan, WU Zu-Hui, CHEN Ji-Yao et al 2013 Chin. Phys. Lett. 30 068702

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Abstract We apply electric fields at different frequencies of 0.1, 1, 10 and 100 kHz to the rat basophilic leukemia (RBL) mast cells in calcium-containing or calcium-free buffers. The stimuli cause changes of the intracellular calcium ion concentration [Ca²⁺]_i as well as the histamine. The [Ca²⁺]_i increases when the frequency of the external electric field increases from 100 Hz to 10 kHz, and then decreases when the frequency further increases from 10 kHz to 100 kHz, showing a peak at 100 kHz. A similar frequency dependence of the histamine release is also found. The [Ca²⁺]_i and the histamine releases at 100 Hz are about the same as the values of the control group with no electrical stimulation. The ruthenium red (RR), an inhibitor to the TRPV (transient receptor potential (TRP) family V) channels across the cell membrane, is used in the experiment to check whether the electric field stimuli act on the TRPV channels. Under an electric field of 10 kHz, the [Ca²⁺]_i in a calcium-concentration buffer is about 3.5 times as much as that of the control group with no electric stimulation, while the [Ca²⁺]_i in a calcium-free buffer is only about 2.2 times. Similar behavior is also found for the histamine release. RR blockage effect on the [Ca²⁺]_i decrease is statistically significant (～75%) when mast cells in the buffer with calcium are stimulated with a 10 kHz electric field in comparison with the result without the RR treatment. This proves that TRPVs are the channels that calcium ions inflow through from the extracellular environment under electrical stimuli. Under this condition, the histamine is also released following a similar way. We suggest that, as far as an electric stimulation is concerned, an application of ac electric field of 10 kHz is better than other frequencies to open TRPV channels in mast cells, and this would cause a significant calcium influx resulting in a significant histamine release, which could be one of the mechanisms for electric therapy.

Received: 19 March 2013 Published: 31 May 2013

PACS:	87.50.C-	(Static and low-frequency electric and magnetic fields effects)
	87.16.Xa	(Signal transduction and intracellular signaling)

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https://cpl.iphy.ac.cn/10.1088/0256-307X/30/6/068702 OR https://cpl.iphy.ac.cn/Y2013/V30/I6/068702

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	ZHU Dan
	WU Zu-Hui
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