| CROSS-DISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY |
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A Label-Free Detection of Biomolecules Using Micromechanical Biosensors |
| Meisam Omidi1**, M. A. Malakoutian2, Mohammadmehdi Choolaei3, F. Oroojalian1, F. Haghiralsadat1, F. Yazdian1 |
1Faculty of New Science and Technology, University of Tehran, Tehran, Iran
2Department of Electrical and Computer Engineering, University of Tehran, Tehran, Iran
3Department of Chemical Engineering, South Tehran Branch, Islamic Azad University, Tehran, Iran
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| Cite this article: |
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Meisam Omidi, M. A. Malakoutian, Mohammadmehdi Choolaei et al 2013 Chin. Phys. Lett. 30 068701 |
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Abstract A Microcantilevers resonator is used to detect a protein biomarker called prostate specific antigen (PSA), which is associated with prostate cancer. Different concentrations of PSA in a buffer solution are detected as a function of deflection of the beams. For this purpose, we use a surface micromachined, antibody-coated polycrystalline silicon micromechanical cantilever beam. Cantilevers have mass sensitivities of the order of 10–17 g/Hz, which result from their small mass. This matter allows them to detect an immobilized antibody monolayer corresponding to a mass of about 70 fg. With these devices, concentrations as low as 150 fg/mL, or 4.5 fM, could be detected from the realistic samples.
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Received: 28 February 2013
Published: 31 May 2013
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| PACS: |
87.85.fk
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(Biosensors)
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87.85.Ox
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(Biomedical instrumentation and micro-electro-mechanical systems (MEMS))
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87.85.Va
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(Micromachining)
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