| CONDENSED MATTER: ELECTRONIC STRUCTURE, ELECTRICAL, MAGNETIC, AND OPTICAL PROPERTIES |
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Label-Free and High-Throughput Detection of Protein Microarrays by Oblique-Incidence Reflectivity Difference Method |
| WANG Xu1,2, LU Heng1, WEN Juan1, YUAN Kun1, LÜ Hui-Bin1, JIN Kui-Juan1, ZHOU Yue-Liang1, YANG Guo-Zhen1,
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1Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190
2School of Science, Jiangnan University, Wuxi 214122
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| Cite this article: |
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WANG Xu, LU Heng, WEN Juan et al 2010 Chin. Phys. Lett. 27 107801 |
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Abstract We label-free detected the biological process of preparing a microarray that includes 400 spots of mouse immunoglobulin G (IgG) as well as the specific hybridization between mouse IgG and goat anti-mouse IgG by an oblique-incidence reflectivity difference (OI-RD) method. The detection results after each process including printing, washing, blocking, and hybridization, demonstrate that the OI-RD method can trace the preparation process of a microarray and detect the specific hybridization between antigens and antibodies. OI-RD is a promising method for label-free and high-throughput detection of biological microarrays.
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| Keywords:
78.20.Ci
42.79.Ls
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Received: 25 January 2010
Published: 26 September 2010
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| PACS: |
78.20.Ci
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(Optical constants (including refractive index, complex dielectric constant, absorption, reflection and transmission coefficients, emissivity))
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42.79.Ls
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(Scanners, image intensifiers, and image converters)
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