Chin. Phys. Lett.  2006, Vol. 23 Issue (4): 1032-1033    DOI:
Original Articles |
Applications of Oblique-Incidence Reflectivity Difference Method in Primary Study of Protein Biomolecules
ZHANG Hong-Yan1;LIANG Ru-Qiang2;JIN Kui-Juan1;Lü Hui-Bin1;ZHU Xiang-Dong3;ZHOU Yue-Liang1;RUAN Kang-Cheng2;YANG Guo-Zhen1
1Laboratory of Optical Physics, Institute of Physics and Center for Condensed Mater Physics, Chinese Academy of Sciences, Beijing 100080 2Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031 3Department of Physics, NSF Center for Biophotonics Science and Technology (CBST), University of California at Davis, California 95817, USA
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ZHANG Hong-Yan, LIANG Ru-Qiang, JIN Kui-Juan et al  2006 Chin. Phys. Lett. 23 1032-1033
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Abstract Oblique-incidence reflectivity difference (OI-RD) analysis is applied to detect the immunoglobulin-G and cytochrome biomolecules on standard glass substrates without fluorescence labelling. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential applications in detection of biochemical processes.
Keywords: 81.70.-q      87.80.+s      87.15.-v      78.20.Wc     
Published: 01 April 2006
PACS:  81.70.-q (Methods of materials testing and analysis)  
  87.80.+s  
  87.15.-v (Biomolecules: structure and physical properties)  
  78.20.Wc  
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https://cpl.iphy.ac.cn/       OR      https://cpl.iphy.ac.cn/Y2006/V23/I4/01032
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ZHANG Hong-Yan
LIANG Ru-Qiang
JIN Kui-Juan
Lü Hui-Bin
ZHU Xiang-Dong
ZHOU Yue-Liang
RUAN Kang-Cheng
YANG Guo-Zhen
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