Measurement of Breaking Force of Fluorescence Labelled Microtubules with Optical Tweezers

Chin. Phys. Lett.

2005, Vol. 22

Issue (5): 1278-1280 DOI:

Original Articles

Measurement of Breaking Force of Fluorescence Labelled Microtubules with Optical Tweezers

LIU Chun-Xiang¹;GUO Hong-Lian¹;XU Chun-Hua²;YUAN Ming²;LI Zhao-Lin¹;CHENG Bing-Ying¹;ZHANG Dao-Zhong¹

¹Optical Physics Laboratory, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 100080 ²State Key Laboratory of Plant Physiology and Biochemistry; College of Biological Sciences,China Agricultural University, Beijing 100094

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LIU Chun-Xiang, GUO Hong-Lian, XU Chun-Hua et al 2005 Chin. Phys. Lett. 22 1278-1280

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Abstract Under illumination of excitation light, the force that can make fluorescent dye-labelled microtubules break up is measured by using dual-beam optical tweezers. It is found that this force is about several piconewtons, which is two orders of magnitude smaller than that without fluorescence label. Microtubules can be elongated about 20% and the increase of the tensile force is nonlinear with the microtubule elongation. Some qualitative explanations are given for the mechanisms about the breakup and elongation of microtubules exposed to excitation light.

Keywords: 87.80.Cc 87.15.He

Published: 01 May 2005

PACS:	87.80.Cc	(Optical trapping)
	87.15.He

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https://cpl.iphy.ac.cn/ OR https://cpl.iphy.ac.cn/Y2005/V22/I5/01278

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	LIU Chun-Xiang
	GUO Hong-Lian
	XU Chun-Hua
	YUAN Ming
	LI Zhao-Lin
	CHENG Bing-Ying
	ZHANG Dao-Zhong

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