| ATOMIC AND MOLECULAR PHYSICS |
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Synchronous Measurement of Ultrafast Anisotropy Decay of the B850 in Bacterial LH2 Complex |
| WANG Yun-Peng, DU Lu-Chao, ZHU Gang-Bei, WANG Zhuan, WENG Yu-Xiang** |
Laboratory of Soft Matter Physics, and Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190
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| Cite this article: |
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WANG Yun-Peng, DU Lu-Chao, ZHU Gang-Bei et al 2015 Chin. Phys. Lett. 32 023101 |
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Abstract Ultrafast anisotropic decay is a prominent parameter revealing ultrafast energy and electron transfer; however, it is difficult to be determined reliably owing to the requirement of a simultaneous availability of the parallel and perpendicular polarized decay kinetics. Nowadays, any measurement of anisotropic decay is a kind of approach to the exact simultaneity. Here we report a novel method for a synchronous ultrafast anisotropy decay measurement, which can well determine the anisotropy, even at a very early time, as the rising phase of the excitation laser pulse. The anisotropic decay of the B850 in bacterial light harvesting antenna complex LH2 of Rhodobacter sphaeroides in solution at room temperature with coherent excitation is detected by this method, which shows a polarization response time of 30 fs, and the energy transfer from the initial excitation to the bacteriochlorophylls in B850 ring takes about 70 fs. The anisotropic decay that is probed at the red side of the absorption spectrum, such as 880 nm, has an initial value of 0.4, corresponding to simulated emission, while the blue side with an anisotropy of 0.1 contributes to the ground-state bleaching. Our results show that the coherent excitation covering the whole ring might not be realized owing to the symmetry breaking of LH2: from C9 symmetry in membrane to C2 symmetry in solution.
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Published: 20 January 2015
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| PACS: |
31.15.at
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(Molecule transport characteristics; molecular dynamics; electronic structure of polymers)
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42.65.Re
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(Ultrafast processes; optical pulse generation and pulse compression)
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78.47.jp
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(Optical nutation)
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[1] Trissl H, Law C J and Cogdell R J 1999 Biochim. Biophys. Acta Bioenerg. 1412 149
[2] Herek J L, Fraser N J, Pullerits T, Martinsson P and Polívka T 2000 Biophys. J. 78 2590
[3] Zazubovich V, Jankowiak R and Small G J 2002 J. Lumin. 98 123
[4] Polli D, Cerullo G, Lanzani G, De Silvestri S, Hashimoto H and Cogdell R J 2006 Biophys. J. 90 2486
[5] Hildner R, Brinks D, Nieder J B, Cogdell R J and van Hulst N F 2013 Science 340 1448
[6] Fidler A F, Singh V P, Long P D, Dahlberg P D and Engel G S 2013 J. Chem. Phys. 139 155101
[7] McDermott G, Prince S M, Freer A A, Hawthornthwaite-Lawless A M, Papiz M Z, Cogdell R J and Isaacs N W 1995 Nature 374 517
[8] Nagarajan V, Alden R G, Williams J C and Parson W W 1996 Proc. Natl. Acad. Sci. USA 93 13774
[9] Sauer K, Cogdell R J, Prince S M, Freer A, Isaacs N W and Scheer H 1996 Photochem. Photobiol. 64 564
[10] Wu H M, Savikhin S, Reddy N R S, Jankowiak R, Cogdell R J, Struve W S and Small G J 1996 J. Phys. Chem. 100 12022
[11] Scheuring S, Francia F, Busselez J, Melandri B A, Rigaud J L and L évy D 2003 J. Biol. Chem. 279 3620
[12] Walz T, Jamieson S J, Bowers C M, Bullough P A, Hunter C N and Bank W 1998 J. Mol. Biol. 282 833
[13] Nagarajan V, Johnson E T, Williams J C and Parson W W 1999 J. Phys. Chem. B 103 2297
[14] Pullerits T, Chachisvilis M and Sundstro V 1996 J. Phys. Chem. 100 10787
[15] Trinkunas G, Herek J, Polívka T, Sundstr?m V and Pullerits T 2001 Phys. Rev. Lett. 86 4167
[16] Kennis J T M, Streltsov A M, Permentier H and Aartsma T J 1997 J. Phys. Chem. B 101 8369
[17] Guo L, Liu Y, Yang Y, Mi J, Xu C, Xu C and Qian S 2002 FEBS Lett. 511 69
[18] Book L D, Ostafin a E, Ponomarenko N, Norris J R and Scherer N F 2000 J. Phys. Chem. B 104 8295
[19] Ma Y, Cogdell R J and Gillbro T 1997 J. Phys. Chem. B 101 1087
[20] Chen X H, Zhang L, Weng Y X, Du L C, Ye M P, Yang G Z, Fujii R, Rondonuwu F S, Koyama Y, Wu Y S and Zhang J P 2005 Biophys. J. 88 4262
[21] Hong X, Weng Y and Li M 2004 Biophys. J. 86 1082
[22] Du L, Weng Y, Hong X, Xian D and Kobayashi K 2006 Chin. Phys. Lett. 23 1861
[23] Matsushita M, Ketelaars M, van Oijen a M, K?hler J, Aartsma T J and Schmidt J 2001 Biophys. J. 80 1604
[24] Bose S 1963 Bacterial Photosynthesis edn: H Gest, A San Pietro and L P Vernon (Ohio: Antioch Press)
[25] Ohashi N, KoChi N, Kuki M, Shimamura T, Cogdell R J and Koyama Y 1998 Biospectroscopy 2 59
[26] Jimenez R, Dikshit S N, Bradforth S E and Fleming G R 1996 J. Phys. Chem. 100 6825 |
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