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A New Method for Determining Dipole Moment Orientation of Single Molecules |
| WANG Chen;LIU Li;WANG Gui-Ying;XU Zhi-Zhan |
| Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800 |
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| Cite this article: |
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WANG Chen, LIU Li, WANG Gui-Ying et al 2004 Chin. Phys. Lett. 21 843-845 |
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Abstract A new imaging method is proposed to determine the three-dimensional dipole moment orientation of single fluorophore. Far-field microscopy can provide orientational information projected in the sample plane, while total internal reflection fluorescence microscopy (TIRFM) can offer the knowledge perpendicular to the surface because longitudinal electric-field components can be generated in total internal reflection geometry. By comparing fluorescence intensities measured with far-field epi-fluorescence microscopy and TIRFM, the exact information of single-fluorescent-molecule orientation is extracted. Detailed analysis of the method is given with a numerical example.
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| Keywords:
42.25.Ja
33.70.Ca
07.60.Pb
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Published: 01 May 2004
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| PACS: |
42.25.Ja
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(Polarization)
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33.70.Ca
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(Oscillator and band strengths, lifetimes, transition moments, and Franck-Condon factors)
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07.60.Pb
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(Conventional optical microscopes)
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Abstract
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