Diffusion in Laser Gradient Field Studied by Fluorescence Correlation Spectroscopy

Chin. Phys. Lett.

2004, Vol. 21

Issue (4): 760-763 DOI:

Original Articles

Diffusion in Laser Gradient Field Studied by Fluorescence Correlation Spectroscopy

MENG Fan-Bo¹;CHEN Bo¹;LIU Guang¹;MA Hui^1,2

¹Molecular and Nano Science Laboratory, Department of Physics, Tsinghua University, Beijing 100084 ²Graduate School at Shenzhen, Tsinghua University, Shenzhen 518057

Cite this article:

MENG Fan-Bo, CHEN Bo, LIU Guang et al 2004 Chin. Phys. Lett. 21 760-763

Download: PDF(308KB)
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks

Abstract We discuss the effects of a laser gradient field on particle diffusion in solvent. A tightly focused laser beam may change both the local concentration and the diffusion time obtained in fluorescence correlation spectroscopy (FCS). A quantitative relation between the gradient field strength and the local particle concentration has been derived. Using the data generated by Monte Carlo simulations, we demonstrate that the quantitative relation can be used to obtain number density and polarizability of the particles by FCS experiments in the laser gradient field. A nonlinear growth of diffusion time with laser power is also shown by both the theoretical analysis and the simulations.

Keywords: 87.80.Cc 87.64.Tt 87.64.Ni 87.64.Aa

Published: 01 April 2004

PACS:	87.80.Cc	(Optical trapping)
	87.64.Tt
	87.64.Ni
	87.64.Aa	(Computer simulation)

TRENDMD:

URL:

https://cpl.iphy.ac.cn/ OR https://cpl.iphy.ac.cn/Y2004/V21/I4/0760

Service
	E-mail this article
	E-mail Alert
	RSS
Articles by authors
	MENG Fan-Bo
	CHEN Bo
	LIU Guang
	MA Hui

Related articles from Frontiers Journals

[1]	DOU Quan-Tao, ZUO Guang-Hong, FANG Hai-Ping. Interaction between a Functionalized Single-Walled Carbon Nanotube and the YAP65WW Protein Domain: a Molecular Dynamics Simulation Study[J]. Chin. Phys. Lett., 2012, 29(6): 760-763
[2]	LIN Hui, ZHANG Jian . Estimating RNA Loop Entropies Using a New Nucleobase Model and Sequential Monte Carlo Method[J]. Chin. Phys. Lett., 2011, 28(8): 760-763
[3]	LI Xue-Cong, SUN Xiu-Dong, LIU Hong-Peng, ZHANG Jian-Long. Light-Induced Agglomeration and Diffusion of Different Particles with Optical Tweezers[J]. Chin. Phys. Lett., 2010, 27(9): 760-763
[4]	REN Yu-Xuan, WU Jian-Guang, CHEN Man, LI Huang, LI Yin-Mei,. Stability of Novel Time-Sharing Dual Optical Tweezers Using a Rotating Tilt Glass Plate[J]. Chin. Phys. Lett., 2010, 27(2): 760-763
[5]	ZHANG Jian, ZHANG Yu-Jie, WANG Wei. An RNA Base Discrete State Model toward Tertiary Structure Prediction[J]. Chin. Phys. Lett., 2010, 27(11): 760-763
[6]	YAO Bao-Li, YAN Shao-Hui, YE Tong, ZHAO Wei. Optical Trapping of Double-Ring Radially Polarized Beam with Improved Axial Trapping Efficiency[J]. Chin. Phys. Lett., 2010, 27(10): 760-763
[7]	JIANG Lai-Dong, DAI Qiao-Feng, FENG Tian-Hua, LIU Jin, WU Li-Jun, LAN Sheng, A. V. Gopal, V. A. Trofimov. In-Situ Characterization of Three-Dimensional Optical Matters by Light Diffraction[J]. Chin. Phys. Lett., 2009, 26(7): 760-763
[8]	ZHOU Jin-Hua, TAO Run-Zhe, HU Zhi-Bin, ZHONG Min-Cheng, WANG Zi-Qiang, CAI Jun, LI Yin-Mei,. Manipulation of Nanoparticles Using Dark-Field-Illumination Optical Tweezers with Compensating Spherical Aberration[J]. Chin. Phys. Lett., 2009, 26(6): 760-763
[9]	MENG Bing-Huan, ZHOU Jin-Hua, ZHONG Min-Cheng, LI Yin-Mei, WU Jian-Guang, REN Hong-Liang. Improvement of Transverse Trapping Efficiency of Optical Tweezers[J]. Chin. Phys. Lett., 2008, 25(6): 760-763
[10]	ZHOU Jin-Hua, QU Lian-Jie, Yao Kun, ZHONG Min-Cheng, LI Yin-Mei. Observing Nanometre Scale Particles with Light Scattering for Manipulation Using Optical Tweezers[J]. Chin. Phys. Lett., 2008, 25(1): 760-763
[11]	XU Chun-Hua, GUO Hong-Lian, QU E, LI Zhao-Lin, YUAN Ming, CHENGBing-Ying, ZHANG Dao-Zhong. Measurement of Binding Force between Microtubule-Associated Protein and Microtubule[J]. Chin. Phys. Lett., 2007, 24(9): 760-763
[12]	ZUO Guang-Hong, HU Jun, FANG Hai-Ping,. Protein Folding under Mediation of Ordering Water: an Off-Lattice Go^--Like Model Study[J]. Chin. Phys. Lett., 2007, 24(8): 760-763
[13]	LIU Hong-Rong, YANG Qi-Bin, CHENG Ling-Peng, ZENG Song-Jun, CAI Can-Ying. Three-Dimensional Reconstruction of Icosahedral Virus by Symmetry-Adapted Functions[J]. Chin. Phys. Lett., 2007, 24(6): 760-763
[14]	ZUO Guang-Hong, ZHANG Jian, WANG Jun, WANG Wei. Folding Behaviour for Proteins BBL and E3BD with Go-like Models[J]. Chin. Phys. Lett., 2005, 22(7): 760-763
[15]	LIU Chun-Xiang, GUO Hong-Lian, XU Chun-Hua, YUAN Ming, LI Zhao-Lin, CHENG Bing-Ying, ZHANG Dao-Zhong. Measurement of Breaking Force of Fluorescence Labelled Microtubules with Optical Tweezers[J]. Chin. Phys. Lett., 2005, 22(5): 760-763

Viewed

Full text

Abstract