Chin. Phys. Lett.  2014, Vol. 31 Issue (12): 127801    DOI: 10.1088/0256-307X/31/12/127801
CONDENSED MATTER: ELECTRONIC STRUCTURE, ELECTRICAL, MAGNETIC, AND OPTICAL PROPERTIES |
Detecting Cells with the Scatter Plot Pattern of an Orthogonal Scattering Mueller Matrix
WANG Qing-Hua**, LI Zhen-Hua, LAI Jian-Cheng, HE An-Zhi
Department of Information Physics and Engineering, Nanjing University of Science and Technology, Nanjing 210094
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WANG Qing-Hua, LI Zhen-Hua, LAI Jian-Cheng et al  2014 Chin. Phys. Lett. 31 127801
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Abstract We investigate the characteristics of the elements of an orthogonal scattering Mueller matrix for spherical cells. Two parameter scatter plots with x- and y-coordinates determined by s11+s12 and s11?s12 values are drawn. The unexpected patterns of these two parameter scatter plots are more sensitive to the relative refractive index of cells. When a series of scatter plots in a database for cells' light scattering are prepared in advance, an image correlation technique can be used to judge the patterns of scatter plots, which shows a promising approach to reveal the tiny change of cells in a flow cytometry.
Published: 12 January 2015
PACS:  78.35.+c (Brillouin and Rayleigh scattering; other light scattering)  
  42.25.Ja (Polarization)  
  87.57.N- (Image analysis)  
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https://cpl.iphy.ac.cn/10.1088/0256-307X/31/12/127801       OR      https://cpl.iphy.ac.cn/Y2014/V31/I12/127801
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WANG Qing-Hua
LI Zhen-Hua
LAI Jian-Cheng
HE An-Zhi
[1] Givan A L 2001 Flow Cytometry: First Principles (New York: Wiley)
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[3] Wang Q H, Li Z H, Lai J C, Zhang Y Y and He A Z 2006 Chin. Phys. Lett. 23 3366
[4] Bohren C F 1983 Absorption and Scattering of Light by Small Particles (New York: Wiley)
[5] Doornbos R M P, Hoekstra A G, Deurloo K E I, De Grooth B G and Sloot P M A 1994 Cytometry 16 236
[6] Evangelidis G D and Psarakis E Z 2008 IEEE Trans. Pattern Anal. Mach. Intell. 30 1858
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