| FUNDAMENTAL AREAS OF PHENOMENOLOGY(INCLUDING APPLICATIONS) |
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Simulation of Far-Field Superresolution Fluorescence Imaging with Two-Color One-Photon Excitation of Reversible Photoactivatable Protein |
| WANG Chen**, QIAO Ling-Ling, MAO Zheng-Le
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State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800
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| Cite this article: |
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WANG Chen, QIAO Ling-Ling, MAO Zheng-Le 2011 Chin. Phys. Lett. 28 054209 |
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Abstract We propose to achieve far-field super-resolution imaging by using offset two-color one-photon (2C1P) excitation of reversible photoactivatable fluorescence proteins. Due to the distinctive photoswitching performance of the proteins, such as dronpa, the fluorescence emission will only come from the overlapped region of activation beam and excitation beam. The analysis solution of rate equation shows that the resolution of offset 2C1P microscope is "engineered" by laser power of excitation and activation beams and the power ratio between them. Superior lateral and transverse resolution is theoretically demonstrated compared with conventional fluorescence scanning microscopy.
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42.30.-d
42.62.Be
87.64.-t
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Received: 26 December 2010
Published: 26 April 2011
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| PACS: |
42.30.-d
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(Imaging and optical processing)
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42.62.Be
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(Biological and medical applications)
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87.64.-t
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(Spectroscopic and microscopic techniques in biophysics and medical physics)
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