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Effects of Substrate Hydrophobicity/Hydrophilicity on Height Measurement of Individual DNA Molecules |
WANG Hua-Bin 1,5;ZHOU Xing-Fei 2;AN Hong-Jie 3, GUO Yun-Chang 1;SUN
Jie-Lin 4;ZHANG Yi 1; HU Jun 1,4 |
| 1Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 2018002Department of Physics, Ningbo University, Ningbo 3152113Tianjin University of Science and Technology, Tianjin 3000404Bio-X Life Science Research Center, Shanghai Jiao TongUniversity, Shanghai 2000305Graduate School of the Chinese Academy of Sciences, Beijing 100080 |
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| Cite this article: |
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WANG Hua-Bin, ZHOU Xing-Fei, AN Hong-Jie et al 2007 Chin. Phys. Lett. 24 644-647 |
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Abstract Effects of substrate hydrophobicity/hydrophilicity on height measurement
of individual ds-DNA molecules are investigated with tapping mode atomic
force microscopy (TMAFM) and vibrating mode scanning polarization force
microscopy (VSPFM). The measured heights of ds-DNA on hydrophobic highly
oriented pyrolytic graphite (HOPG) are remarkably less than those on
hydrophilic bare mica and Ni 2+ treated mica in both TMAFM and VSPFM. By analysing the results, we propose that the hydrophobicity/hydrophilicity of substrate can greatly influence the height measurement of DNA molecules.
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| Keywords:
06.60.Ei
07.79.Lh
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Received: 30 October 2006
Published: 08 February 2007
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