Reversible Conformational Changes of PsbO Protein Detected by Terahertz Time-Domain Spectroscopy
CHEN Hua1, CHEN Gui-Ying2, LI Shu-Qin2, WANG Li3
1Department of Physics, Southeast University, Nanjing 2111892Photosynthesis Research Center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 1000933Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190
Reversible Conformational Changes of PsbO Protein Detected by Terahertz Time-Domain Spectroscopy
CHEN Hua1, CHEN Gui-Ying2, LI Shu-Qin2, WANG Li3
1Department of Physics, Southeast University, Nanjing 2111892Photosynthesis Research Center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 1000933Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190
摘要We used a terahertz time-domain spectroscope (THz-TDS) to detect the reversible conformational changes of PsbO protein induced by N-bromosuccinimide and Guanidine Hydrochloride. The veracity and sensitivity are confirmed by the fluorescence emission spectra. The results demonstrate that THz-TDS has both advantages and disadvantages in monitoring the denaturation process of proteins, which is important in applying THz-TDS technique to studying biomolecules.
Abstract：We used a terahertz time-domain spectroscope (THz-TDS) to detect the reversible conformational changes of PsbO protein induced by N-bromosuccinimide and Guanidine Hydrochloride. The veracity and sensitivity are confirmed by the fluorescence emission spectra. The results demonstrate that THz-TDS has both advantages and disadvantages in monitoring the denaturation process of proteins, which is important in applying THz-TDS technique to studying biomolecules.
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2009, Vol. 26 
