Multi-exponentially Photoelectric Response of Bacteriorhodopsin
YAO Bao-Li1 , XU Da-Lun1, HOU Xun1, HU Kun-Sheng2, WANG Ao-Jin2
1State Key Laboratory of Transient Optics and Technology, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an 710068
2Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101
Multi-exponentially Photoelectric Response of Bacteriorhodopsin
YAO Bao-Li1 ;XU Da-Lun1;HOU Xun1;HU Kun-Sheng2;WANG Ao-Jin2
1State Key Laboratory of Transient Optics and Technology, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an 710068
2Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101
Abstract： A thin oriented bacteriorhodopsin (bR) film is deposited on a stainless steel slide by use of the electrophoretic sedimentation method. A junction is made with electrolyte gels having a counterelectrode to construct a bR-based photoelectric detector. The photoelectric response signal to a 10ns laser pulse is measured. A theory on the photoelectric kinetics of bR is developed based on the concept of the charge displacement current and the bR photocycle rate equations. Comparison between the theoretical and experimental results proves that the bR photoelectric response to a short laser pulse is a multi-exponential process. The decay time constants and amplitudes of each exponential component are obtained by data fitting.
2001, Vol. 18 
