Automatic Compensation of Total Phase Aberrations in Digital Holographic Biological Imaging
ZHANG Yi-Zhuo1,2, WANG Da-Yong1,2**, WANG Yun-Xin1, TAO Shi-Quan1
1College of Applied Sciences, Beijing University of Technology, Beijing 100124 2Institute of Information Photonics Technology, Beijing University of Technology, Beijing 100124
Automatic Compensation of Total Phase Aberrations in Digital Holographic Biological Imaging
ZHANG Yi-Zhuo1,2, WANG Da-Yong1,2**, WANG Yun-Xin1, TAO Shi-Quan1
1College of Applied Sciences, Beijing University of Technology, Beijing 100124 2Institute of Information Photonics Technology, Beijing University of Technology, Beijing 100124
摘要Digital holographic microscopy has been a powerful metrological technique for phase-contrast imaging. However inherent phase aberrations always exist and degrade the quality of the phase-contrast images. A surface fitting method based on an improved mathematic model is proposed, which can be used to remove the phase aberrations without any pre-knowledge of the setup or manual operation. The improved mathematic model includes not only the usual terms but also the cross terms and the high order terms to describe the phase aberrations with high accuracy. Meanwhile, a non-iterative algorithm is used to solve the parametersand thus less computational load is imposed. The proposed method is applied to the live imaging of cells. The experimental results verify its validity.
Abstract：Digital holographic microscopy has been a powerful metrological technique for phase-contrast imaging. However inherent phase aberrations always exist and degrade the quality of the phase-contrast images. A surface fitting method based on an improved mathematic model is proposed, which can be used to remove the phase aberrations without any pre-knowledge of the setup or manual operation. The improved mathematic model includes not only the usual terms but also the cross terms and the high order terms to describe the phase aberrations with high accuracy. Meanwhile, a non-iterative algorithm is used to solve the parametersand thus less computational load is imposed. The proposed method is applied to the live imaging of cells. The experimental results verify its validity.
ZHANG Yi-Zhuo;WANG Da-Yong;**;WANG Yun-Xin;TAO Shi-Quan
. Automatic Compensation of Total Phase Aberrations in Digital Holographic Biological Imaging[J]. 中国物理快报, 2011, 28(11): 114209-114209.
ZHANG Yi-Zhuo, WANG Da-Yong, **, WANG Yun-Xin, TAO Shi-Quan
. Automatic Compensation of Total Phase Aberrations in Digital Holographic Biological Imaging. Chin. Phys. Lett., 2011, 28(11): 114209-114209.
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2011, Vol. 28 
