Correlation of Photosensitization and Binding Mode of Methylene Blue and DNA
LIU Tao, ZHANG Feng, CHEN Ping, TANG Guo-Qing, LIN Lie**
Key Laboratory of Optoelectronic Information Science and Technology (Ministry of Education), Institute of Modern Optics, Nankai University, Tianjin 300071
Correlation of Photosensitization and Binding Mode of Methylene Blue and DNA
LIU Tao, ZHANG Feng, CHEN Ping, TANG Guo-Qing, LIN Lie**
Key Laboratory of Optoelectronic Information Science and Technology (Ministry of Education), Institute of Modern Optics, Nankai University, Tianjin 300071
摘要Binding of methylene blue (MB) and DNA, photosensitization of MB in DNA, and correlation of photosensitization and binding mode are studied at different concentration ratios of DNA and MB. The absorption spectra indicate that the electrostatic binding is the main mode at low γ ratios (γ≤2), while at high γ ratios (γ>2) the intercalative binding is dominated. Studies on dynamics of photosensitization formation of singlet oxygen (1O2) for MB in DNA are carried out by using time−resolved technology. There are no obvious changes of the singlet oxygen lifetime and the triplet state MB molecule (MB3+∗) lifetime at low ratios, they are about 4 μs and 1 µs, respectively. However, we could not obtain the 1O2 lifetime and MB3+∗ lifetime due to the great decrease of 1O2 phosphorescence signals at high ratios. These results show that the photosensitization and binding mode of MB in DNA possess high correlation. When MB binds with DNA by electrostatic interaction, type−II photosensitization of MB plays a major role in photodynamic effect, the damage of DNA probably could be ascribed to 1O2. However, at high ratios, binding mode between MB and DNA turns to intercalative binding, which greatly weakens the type−II photosensitization process. Charge transfer between MB and DNA possibly becomes the main damage mechanism.
Abstract:Binding of methylene blue (MB) and DNA, photosensitization of MB in DNA, and correlation of photosensitization and binding mode are studied at different concentration ratios of DNA and MB. The absorption spectra indicate that the electrostatic binding is the main mode at low γ ratios (γ≤2), while at high γ ratios (γ>2) the intercalative binding is dominated. Studies on dynamics of photosensitization formation of singlet oxygen (1O2) for MB in DNA are carried out by using time−resolved technology. There are no obvious changes of the singlet oxygen lifetime and the triplet state MB molecule (MB3+∗) lifetime at low ratios, they are about 4 μs and 1 µs, respectively. However, we could not obtain the 1O2 lifetime and MB3+∗ lifetime due to the great decrease of 1O2 phosphorescence signals at high ratios. These results show that the photosensitization and binding mode of MB in DNA possess high correlation. When MB binds with DNA by electrostatic interaction, type−II photosensitization of MB plays a major role in photodynamic effect, the damage of DNA probably could be ascribed to 1O2. However, at high ratios, binding mode between MB and DNA turns to intercalative binding, which greatly weakens the type−II photosensitization process. Charge transfer between MB and DNA possibly becomes the main damage mechanism.
LIU Tao;ZHANG Feng;CHEN Ping;TANG Guo-Qing;LIN Lie**
. Correlation of Photosensitization and Binding Mode of Methylene Blue and DNA[J]. 中国物理快报, 2011, 28(1): 14208-014208.
LIU Tao, ZHANG Feng, CHEN Ping, TANG Guo-Qing, LIN Lie**
. Correlation of Photosensitization and Binding Mode of Methylene Blue and DNA. Chin. Phys. Lett., 2011, 28(1): 14208-014208.
[1] Qin Y, Pang J Y, Chen W H, Cai Z W and Jiang Z H 2006 Bioorgan. Med. Chem. 14 25
[2] Bhadra K, Maiti M and Kumar G S 2007 Biochimica et Biophysica Acta (BBA): General Subjects 1770 1071
[3] Dan F, Ming W X, Bo L H, Sheng D L, Min H Y, Huan Z and Lin Z S 2008 Acta Chim. Sin. 66 443 (in Chinese)
[4] Zhang L Z and Tang G Q 2004 J. Photoch. Photobiol. B: Biol. 74 119
[5] Tong C, Hu Z and Wu J 2010 J. Fluoresc. 20 261
[6] Rye H S, Dabora J M, Quesada M A, Mathies R A and Glazer A N 1993 Anal. Biochem. 208 144
[7] Rohs R, Sklenar H, Lavery R and Roder B 2000 J. Am. Chem. Soc. 122 2860
[8] Zhao G C, Zhu J J, Zhang J J and Chen H Y 1999 Anal. Chim. Acta 394 337
[9] Zhao G C, Zhu J J and Chen H Y 1999 Spectrochim. Acta A: Mol. Biomol. Spectrosc. 55 1109
[10] Hu Z and Tong C 2007 Anal. Chim. Acta 587 187
[11] Wang Y and Zhou A 2007 J. Photoch. Photobiol. A: Chem. 190 121
[12] Tardivo J P, Del Giglio A, Paschoal L H C, Ito A S and Baptista M S 2004 Photodiagnosis. Photodyn. Ther. 1 345
[13] Gabrielli D, Belisle E, Severino D, Kowaltowski A J and Baptista M S 2004 Photochem. Photobiol. 79 227
[14] Chen Y, Zheng W, Li Y, Zhong J, Ji J and Shen P 2008 Cancer Science 99 2019
[15] Tardivo J P, Del Giglio A, de Oliveira C S, Gabrielli D S, Junqueira H C, Tada D B, Severino D, de Fátima Turchiello R and Baptista M S 2005 Photodiagnosis. Photodyn. Ther. 2 175
[16] Wilkinson F, Helman W P and Ross A B 1995 J. Phys. Chem. Ref. Data 24 663
[17] Long E C and Barton J K 1990 Accounts Chem. Res. 23 271
[18] Krasnovsky Jr A A 2008 J. Photoch. Photobiol. A: Chem. 196 210
[19] Schweitzer C and Schmidt R 2003 Chem. Rev. 103 1685
[20] Skovsen E, Snyder J W, Lambert J D C and Ogilby P R 2005 J. Phys. Chem. B 109 8570
[21] Boon E M, Jackson N M, Wightman M D, Kelley S O, Hill M G and Barton J K 2003 J. Phys. Chem. B 107 11805
[22] Genereux J C and Barton J K 2009 Chem. Rev. 110 1642
[23] Holmlin R E, Dandliker P J and Barton J K 1997 Angew. Chem. Int. Ed. Engl. 36 2714