Pairing Mismatched ssDNA to dsDNA Studied with Reflectometric Interference Spectroscopy Sensor
Qing-Qing Wu, Kai-Ge Wang** , Dan Sun, Shuang Wang, Chen Zhang, Wei Zhao
Institute of Photonics & Photon-Technology, National Center for International Research of Photoelectric Technology & Nano-functional Materials, State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, Key Laboratory of Optoelectronic Technology of Shaanxi Province, Northwest University, Xi'an 710069
Abstract ：The interaction between two single-stranded DNA (ssDNA) molecules as pairing to a double-stranded DNA (dsDNA) molecule is studied by the reflectometric interference spectroscopy (RIFS) technology. A nano-porous anode alumina membrane coated an Au layer is employed as the sensor substrate. The results indicate that when there are mismatched nucleotide bases, the effective optical thicknesses (${\rm OT}_{\rm eff})$ have obvious difference, and the changes of ${\rm OT}_{\rm eff}$ are connected with the sensor layer thickness and the effective refractive index. It is also demonstrated that the RIFS technique can be used to precisely detect the ssDNA molecules with individual base mismatched as pairing to dsDNA.
收稿日期: 2016-04-07
出版日期: 2016-08-31
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2016, Vol. 33 
