Light-Induced Charge Separation and Transfer in Bacteriorhodopsin

The photo-voltage signals in bacteriorhodopsin(bR) excited by 1064 nm pulse laser are different from those by 532 or 355nm. It shows that the positive and negative photoelectric signals are produced by the motion of the positive and negative charges, respectively, and more energy is needed for producing the positive charges than the negative. The mechanism of light-induced charge generation and charge transfer in bR was studied and analyzed by measuring the photoelectric signals with different impedance of measuring circuit and different pulse-width of 532nm laser as pump light.