High Optical Magnification Three-Dimensional Integral Imaging of Biological Micro-organism

A high optical magnification three-dimensional imaging system is proposed using an optic microscope whose ocular (eyepiece) is retained and the structure of the transmission mode is not destroyed. The elemental image array is captured through the micro lens array. Due to the front diffuse transmission element, each micro lens sees a slightly different spatial perspective of the scene, and a different independent image is formed in each micro lens channel. Each micro lens channel is imaged by a Fourier lens and captured by a CCD. The design translating the stage in x or y provides no parallax. Compared with the conventional integral imaging of micro-objects, the optical magnification of micro-objects in the proposed system can enhanced remarkably. The principle of the enhancement of the image depth is explained in detail and the experimental results are presented.