Effects of Substrate Hydrophobicity/Hydrophilicity on Height Measurement of Individual DNA Molecules
-
Abstract
Effects of substrate hydrophobicity/hydrophilicity on height measurement
of individual ds-DNA molecules are investigated with tapping mode atomic
force microscopy (TMAFM) and vibrating mode scanning polarization force
microscopy (VSPFM). The measured heights of ds-DNA on hydrophobic highly
oriented pyrolytic graphite (HOPG) are remarkably less than those on
hydrophilic bare mica and Ni 2+ treated mica in both TMAFM and VSPFM. By analysing the results, we propose that the hydrophobicity/hydrophilicity of substrate can greatly influence the height measurement of DNA molecules.
Article Text
-
-
-
About This Article