| GENERAL |
|
|
|
|
Analysis on the DNA Fingerprinting of Aspergillus Oryzae Mutant Induced by High Hydrostatic Pressure |
| WANG Hua1, ZHANG Jian1, YANG Fan2, WANG Kai1, SHEN Si-Le2**, LIU Bing-Bing1, ZOU Bo1**, ZOU Guang-Tian1
|
1State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012
2College of Life Science, Jilin University, Changchun 130012
|
|
| Cite this article: |
|
WANG Hua, ZHANG Jian, YANG Fan et al 2011 Chin. Phys. Lett. 28 010703 |
|
|
|
|
Abstract The mutant strains of aspergillus oryzae (HP300a) are screened under 300 MPa for 20 min. Compared with the control strains, the screened mutant strains have unique properties such as genetic stability, rapid growth, lots of spores, and high protease activity. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) are used to analyze the DNA fingerprinting of HP300a and the control strains. There are 67.9% and 51.3% polymorphic bands obtained by these two markers, respectively, indicating significant genetic variations between HP300a and the control strains. In addition, comparison of HP300a and the control strains, the genetic distances of random sequence and simple sequence repeat of DNA are 0.51 and 0.34, respectively.
|
| Keywords:
07.35.+k
91.60.Gf
87.14.Gk
87.15.Qt
|
|
|
Received: 26 April 2010
Published: 23 December 2010
|
|
|
|
|
|
|
|
[1] Ma Y M, Chen H Y, Li X F, Gao L L, Cui Q L and Zou G T 2007 Chin. Phys. Lett. 24 1180
[2] Lin A L, Wang K, Zhao Y, Hao J and Zou B 2007 Chin. Phys. Lett. 24 3085
[3] Yu Y, Zhai G J and Jin C Q 2009 Chin. Phys. Lett. 26 026201
[4] Hayashi R 2002 Biochim. Biophys. Acta 1595 397
[5] Winter R, Erbes J, Czeslik C and Gabke A 1998 J. Phys: Condens. Matter. 10 11499
[6] Chryssomallis G S, Torgerson P M, Drickamer H G and Weber G 1981 Biochemistry. 20 3955
[7] Tokuda S, Miyazaki H, Nakajima K, Yamada T and Marunaka Y 2009 Biochem. Biophys. Res. Commun. 390 1315
[8] Ando N, Barstow B, Baase W A, Fields A, Matthews B W and Gruner S M 2008 Biochemistry. 47 11097
[9] Winter R 2002 Biochim. Biophys. Acta 1595 160
[10] Shen S L, Wang Z W, Sha X H, Wang H, Li L, Lin X Y, Long L K, Weng K N, Liu B and Zou G T 2006 Sci. China C 49 97
[11] Lin X Y, Long L K, Shan X H, Zhang S Y, Shen S L and Liu B 2006 J. Exp. Bot. 57 2313
[12] Pribenszky Cs, Dub Y and Molnar M 2008 Anim. Reprod. Sci. 106 200
[13] Mañas P and Mackey B M 2004 Appl. Environ. Microb. 70 1545
[14] Domitrovic T, Fernandes C M, Boy-Marcotte E and Kurtenbach E 2006 FEBS Lett. 580 6033
[15] Wang H, Shen S L, Li Z H, Cui H, Wang Y F, Fu X X, Yang F, Chang L, Zheng X Y and Zou G T 2008 China. J. High Pressure Phys. 22 259
[16] Zou Y P, Ge S and Wang X D 2001 Molecular marker of systematic and evolutionary botany (Beijing: Science Press) (in Chinese) p 190
[17] Nei M and Li W H 1979 Proc. Natl. Acad. Sci. USA. 76 5269
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
