| CROSS-DISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY |
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Pairing Mismatched ssDNA to dsDNA Studied with Reflectometric Interference Spectroscopy Sensor |
| Qing-Qing Wu, Kai-Ge Wang**, Dan Sun, Shuang Wang, Chen Zhang, Wei Zhao |
Institute of Photonics & Photon-Technology, National Center for International Research of Photoelectric Technology & Nano-functional Materials, State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, Key Laboratory of Optoelectronic Technology of Shaanxi Province, Northwest University, Xi'an 710069
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Qing-Qing Wu, Kai-Ge Wang, Dan Sun et al 2016 Chin. Phys. Lett. 33 088701 |
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Abstract The interaction between two single-stranded DNA (ssDNA) molecules as pairing to a double-stranded DNA (dsDNA) molecule is studied by the reflectometric interference spectroscopy (RIFS) technology. A nano-porous anode alumina membrane coated an Au layer is employed as the sensor substrate. The results indicate that when there are mismatched nucleotide bases, the effective optical thicknesses (${\rm OT}_{\rm eff})$ have obvious difference, and the changes of ${\rm OT}_{\rm eff}$ are connected with the sensor layer thickness and the effective refractive index. It is also demonstrated that the RIFS technique can be used to precisely detect the ssDNA molecules with individual base mismatched as pairing to dsDNA.
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Received: 07 April 2016
Published: 31 August 2016
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