Hydrogen Sulfide Detection Using a Gold Nanoparticle/Metalloprotein Based Probe
Meisam Omidi1,2, Gh. Amoabediny1,2, F. Yazdian1,2**, M. Habibi-Rezaei3,4
1Faculty of New Science and Technology University of Tehran, Tehran, Iran 2Research Center for New Technologies in Life-Science Engineering, University of Tehran, Tehran, Iran 3School of Biology, College of Science, University of Tehran, Tehran, Iran 4Nanobiomedicine Center of Excellance, University of Tehran, Tehran, Iran
Abstract：We present a simple method for direct detection of hydrogen sulfide (H2S) in an aqueous solution. This method represents a novel biosensor based on metalloprotein cytochrome c (cyt c) with the localized surface plasmon resonance of gold nanoparticles (AuNPs). For this purpose, we develop a new approach based on attaching chemically-modified cyt c onto AuNPs. Here, by reacting H2S with protein heme center, its conformation changes in the locality of the heme moiety. The conformational changes occurring in the protein alter the spectral characteristics by changing the dielectric properties of AuNPs. The conformational changes of cyt c induced by the H2S interaction are characterized by the UV-visible absorption spectroscopy and the circular dichroism technique. The limit of the detection and sensitivity of the AuNPs/cyt c biosensor are evaluated by using UV-visible spectroscopy. According to the experiments, it is revealed that H2S can be detected at a concentration of 4.0 μM (1.3 ppb) by the fabricated AuNPs/cyt c biosensor. In addition, the sensor retains activity and gives reproducible results after storage in 4°C for 60 d. This simple and cost-effective sensing platform provides a rapid and convenient detection for H2S at concentrations far below the hazardous limit.
2014, Vol. 31 
