A Label-Free Detection of Biomolecules Using Micromechanical Biosensors
Meisam Omidi1**, M. A. Malakoutian2, Mohammadmehdi Choolaei3, F. Oroojalian1, F. Haghiralsadat1, F. Yazdian1
1Faculty of New Science and Technology, University of Tehran, Tehran, Iran 2Department of Electrical and Computer Engineering, University of Tehran, Tehran, Iran 3Department of Chemical Engineering, South Tehran Branch, Islamic Azad University, Tehran, Iran
Abstract：A Microcantilevers resonator is used to detect a protein biomarker called prostate specific antigen (PSA), which is associated with prostate cancer. Different concentrations of PSA in a buffer solution are detected as a function of deflection of the beams. For this purpose, we use a surface micromachined, antibody-coated polycrystalline silicon micromechanical cantilever beam. Cantilevers have mass sensitivities of the order of 10–17 g/Hz, which result from their small mass. This matter allows them to detect an immobilized antibody monolayer corresponding to a mass of about 70 fg. With these devices, concentrations as low as 150 fg/mL, or 4.5 fM, could be detected from the realistic samples.
. [J]. 中国物理快报, 2013, 30(6): 68701-068701.
Meisam Omidi, M. A. Malakoutian, Mohammadmehdi Choolaei, F. Oroojalian, F. Haghiralsadat, F. Yazdian . A Label-Free Detection of Biomolecules Using Micromechanical Biosensors. Chin. Phys. Lett., 2013, 30(6): 68701-068701.
2013, Vol. 30 
