Label-Free and Real-Time Detection of Antigen-Antibody Capture Processes Using the Oblique-Incidence Reflectivity Difference Technique
HE Li-Ping 1,2, DAI Jun1, SUN Yue1,2, WANG Jing-Yi1, LÜ Hui-Bin1**, WANG Shu-Fang2**, JIN Kui-Juan1, ZHOU Yue-Liang1, YANG Guo-Zhen1
1Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190 2College of Physics Science and Technology, Hebei University, Baoding 071002
Label-Free and Real-Time Detection of Antigen-Antibody Capture Processes Using the Oblique-Incidence Reflectivity Difference Technique
HE Li-Ping 1,2, DAI Jun1, SUN Yue1,2, WANG Jing-Yi1, LÜ Hui-Bin1**, WANG Shu-Fang2**, JIN Kui-Juan1, ZHOU Yue-Liang1, YANG Guo-Zhen1
1Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190 2College of Physics Science and Technology, Hebei University, Baoding 071002
We successfully label-free and real-time detect the capture processes of human immunoglobulin G (IgG)/goat anti-human IgG and mouse IgG/goat anti-mouse IgG antigen-antibody pairs with different concentrations using the oblique-incidence reflectivity difference (OIRD) method, and obtain the interaction kinetics curves and the interaction times. The experimental results prove that the OIRD method is a promising technique for label-free and real-time detection of the biomolecular interaction processes and achieving the quantitative information of interaction kinetics.
We successfully label-free and real-time detect the capture processes of human immunoglobulin G (IgG)/goat anti-human IgG and mouse IgG/goat anti-mouse IgG antigen-antibody pairs with different concentrations using the oblique-incidence reflectivity difference (OIRD) method, and obtain the interaction kinetics curves and the interaction times. The experimental results prove that the OIRD method is a promising technique for label-free and real-time detection of the biomolecular interaction processes and achieving the quantitative information of interaction kinetics.
HE Li-Ping, DAI Jun, SUN Yue, WANG Jing-Yi, LÜ Hui-Bin, WANG Shu-Fang, JIN Kui-Juan, ZHOU Yue-Liang, YANG Guo-Zhen. Label-Free and Real-Time Detection of Antigen-Antibody Capture Processes Using the Oblique-Incidence Reflectivity Difference Technique[J]. 中国物理快报, 2012, 29(7): 70702-070702.
HE Li-Ping, DAI Jun, SUN Yue, WANG Jing-Yi, LÜ Hui-Bin, WANG Shu-Fang, JIN Kui-Juan, ZHOU Yue-Liang, YANG Guo-Zhen. Label-Free and Real-Time Detection of Antigen-Antibody Capture Processes Using the Oblique-Incidence Reflectivity Difference Technique. Chin. Phys. Lett., 2012, 29(7): 70702-070702.
2012, Vol. 29 
