Label-Free and High-Throughput Detection of Protein Microarrays by Oblique-Incidence Reflectivity Difference Method
WANG Xu1,2, LU Heng1, WEN Juan1, YUAN Kun1, LÜ Hui-Bin1, JIN Kui-Juan1, ZHOU Yue-Liang1, YANG Guo-Zhen1,
1Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190 2School of Science, Jiangnan University, Wuxi 214122
Label-Free and High-Throughput Detection of Protein Microarrays by Oblique-Incidence Reflectivity Difference Method
WANG Xu1,2, LU Heng1, WEN Juan1, YUAN Kun1, LÜ Hui-Bin1, JIN Kui-Juan1, ZHOU Yue-Liang1, YANG Guo-Zhen1,
1Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190 2School of Science, Jiangnan University, Wuxi 214122
摘要We label-free detected the biological process of preparing a microarray that includes 400 spots of mouse immunoglobulin G (IgG) as well as the specific hybridization between mouse IgG and goat anti-mouse IgG by an oblique-incidence reflectivity difference (OI-RD) method. The detection results after each process including printing, washing, blocking, and hybridization, demonstrate that the OI-RD method can trace the preparation process of a microarray and detect the specific hybridization between antigens and antibodies. OI-RD is a promising method for label-free and high-throughput detection of biological microarrays.
Abstract：We label-free detected the biological process of preparing a microarray that includes 400 spots of mouse immunoglobulin G (IgG) as well as the specific hybridization between mouse IgG and goat anti-mouse IgG by an oblique-incidence reflectivity difference (OI-RD) method. The detection results after each process including printing, washing, blocking, and hybridization, demonstrate that the OI-RD method can trace the preparation process of a microarray and detect the specific hybridization between antigens and antibodies. OI-RD is a promising method for label-free and high-throughput detection of biological microarrays.
(Scanners, image intensifiers, and image converters)
引用本文:
WANG Xu;LU Heng;WEN Juan;YUAN Kun;LÜHui-Bin;JIN Kui-Juan;ZHOU Yue-Liang;YANG Guo-Zhen;
. Label-Free and High-Throughput Detection of Protein Microarrays by Oblique-Incidence Reflectivity Difference Method[J]. 中国物理快报, 2010, 27(10): 107801-107801.
WANG Xu, LU Heng, WEN Juan, YUAN Kun, LÜ, Hui-Bin, JIN Kui-Juan, ZHOU Yue-Liang, YANG Guo-Zhen,
. Label-Free and High-Throughput Detection of Protein Microarrays by Oblique-Incidence Reflectivity Difference Method. Chin. Phys. Lett., 2010, 27(10): 107801-107801.
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