Observation of the in-vivo Reporter of Green Fluorescent Protein in a Plant Root by Scanning Near-Field Optical Microscopy
SUN Jia-Lin1, XU Jian-Hua1, CHEN Tao1, TAN Xiao-Jing1, CAO Yang1, LIU Jin-Yuan3, XIE Ai-Fang2, ZHANG Ze-Bo2, GUO Ji-Hua1
1Key Laboratory of Atomic and Molecular Nanosciences of Education Ministry and Department of Physics, Tsinghua University, Beijing 100084
2Laboratory of Optical Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100080
3Department of Life Science and Biotechnology, Tsinghua University, Beijing 100084
Observation of the in-vivo Reporter of Green Fluorescent Protein in a Plant Root by Scanning Near-Field Optical Microscopy
SUN Jia-Lin1;XU Jian-Hua1;CHEN Tao1;TAN Xiao-Jing1;CAO Yang1;LIU Jin-Yuan3;XIE Ai-Fang2;ZHANG Ze-Bo2;GUO Ji-Hua1
1Key Laboratory of Atomic and Molecular Nanosciences of Education Ministry and Department of Physics, Tsinghua University, Beijing 100084
2Laboratory of Optical Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100080
3Department of Life Science and Biotechnology, Tsinghua University, Beijing 100084
Abstract： An in vivo reporter of green-fluorescent protein (GFP) in a living plant root has been imaged by scanning near-field optical microscope (SNOM) in the transmission mode. The exciting light is 488 nm wavelength of the argon ion laser and the bandpass filters (514±10) nm is put into the detecting optical pathway. The results indicate that in the living plant cells, the GFPs gather together and form an area of 2-4μm, rather than being individually distributed. The transmission coefficient of the eigenfunction is incorporated into Bethe's theoretical model modified by Grober, and the near-field excited light intensity along the fibre probe axis (z-axis) in the air medium and biological medium is calculated. Based on that, along the z-axis direction of the GFP detected in the sample, numerous GFPs locate near the epidermal cells wall (in the range of 0-38 nm) in the living root. The experiments show that SNOM has an advantage of optical nanometer-scale resolution along the z-axis.
SUN Jia-Lin;XU Jian-Hua;CHEN Tao;TAN Xiao-Jing;CAO Yang;LIU Jin-Yuan;XIE Ai-Fang;ZHANG Ze-Bo;GUO Ji-Hua. Observation of the in-vivo Reporter of Green Fluorescent Protein in a Plant Root by Scanning Near-Field Optical Microscopy[J]. 中国物理快报, 2002, 19(9): 1389-1391.
SUN Jia-Lin, XU Jian-Hua, CHEN Tao, TAN Xiao-Jing, CAO Yang, LIU Jin-Yuan, XIE Ai-Fang, ZHANG Ze-Bo, GUO Ji-Hua. Observation of the in-vivo Reporter of Green Fluorescent Protein in a Plant Root by Scanning Near-Field Optical Microscopy. Chin. Phys. Lett., 2002, 19(9): 1389-1391.
2002, Vol. 19 
