Femtosecond Time-Resolved Absorption of Oxyhaemoglobin Photolysis in Living Erythrocytes
CEN Yan1,2 , MA Jiong1,2 , ZHU Rong-Yi1,2 , LU Jun-Jun1,2 , QIAN Shi-Xiong1 , CHEN Ji-Yao1,3
1 Department of Physics, Fudan University, Shanghai 200433
2 State Key Laboratory of Applied Surface Physics, Fudan University, Shanghai 200433
3 State Key Laboratory for Advanced Photonic Materials and Devices, Fudan University, Shanghai 200433
Femtosecond Time-Resolved Absorption of Oxyhaemoglobin Photolysis in Living Erythrocytes
CEN Yan1,2 ;MA Jiong1,2 ;ZHU Rong-Yi1,2 ;LU Jun-Jun1,2 ;QIAN Shi-Xiong1 ;CHEN Ji-Yao1,3
1 Department of Physics, Fudan University, Shanghai 200433
2 State Key Laboratory of Applied Surface Physics, Fudan University, Shanghai 200433
3 State Key Laboratory for Advanced Photonic Materials and Devices, Fudan University, Shanghai 200433
关键词 ：
78.47.+p ,
82.53.Eb ,
87.14.Ee
Abstract ： By using the pump--probe technique, the femtosecond (fs) time-resolved absorption dynamics of oxyhaemoglobin (oxy Hb) in living erythrocytes was studied for the first time to our knowledge. The changing course of erythrocyte absorption at 400nm was found to be composed of three exponential decay components with the time constants of 200±28fs, 3.60±0.14ps and 215±21ps respectively, which are consistent with those of oxy Hb water solution measured in the same way, indicating that the fast process (less than 1ns) of photo-induced response in erythrocytes was as the same as that in oxy Hb aqueous solution. These three decay components were assigned to the transitions from excited states of unligated haemoglobin (HbI * , HbII * ) and fast geminate recombination of O2 and Hb, respectively.
Key words ：
78.47.+p
82.53.Eb
87.14.Ee
出版日期: 2004-08-01
引用本文:
CEN Yan;MA Jiong;ZHU Rong-Yi;LU Jun-Jun;QIAN Shi-Xiong;CHEN Ji-Yao;. Femtosecond Time-Resolved Absorption of Oxyhaemoglobin Photolysis in Living Erythrocytes[J]. 中国物理快报, 2004, 21(8): 1636-1639.
链接本文:
https://cpl.iphy.ac.cn/CN/
或
https://cpl.iphy.ac.cn/CN/Y2004/V21/I8/1636
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