摘要We develop a new method to distinguish structural change of cells based on light scattering and Fourier spectra analysis. The light scattering detection system is composed of a laser source, an optical microscope, a CCD with high resolution and low distortion. After the scattering patterns of cells are recorded by the CCD, the Fourier spectra are obtained by the intensity distribution of scattered light. In the experiment, the change of cell structure is designed by sonication treatment. It is found that different typical peaks can be shown in the Fourier spectra of MCF7 cells with and without sonication treatment, which indicates that this method can be used to distinguish the structural change of cells.
Abstract：We develop a new method to distinguish structural change of cells based on light scattering and Fourier spectra analysis. The light scattering detection system is composed of a laser source, an optical microscope, a CCD with high resolution and low distortion. After the scattering patterns of cells are recorded by the CCD, the Fourier spectra are obtained by the intensity distribution of scattered light. In the experiment, the change of cell structure is designed by sonication treatment. It is found that different typical peaks can be shown in the Fourier spectra of MCF7 cells with and without sonication treatment, which indicates that this method can be used to distinguish the structural change of cells.
(Spatiotemporal pattern formation in cellular populations)
引用本文:
JIN Yong-Long;YANG Fang;WANG Meng;ZHANG Yu;GU Ning. Distinguishing Structure Change of Cells Based on Analysis of Light Scattering Patterns[J]. 中国物理快报, 2008, 25(11): 4013-4015.
JIN Yong-Long, YANG Fang, WANG Meng, ZHANG Yu, GU Ning. Distinguishing Structure Change of Cells Based on Analysis of Light Scattering Patterns. Chin. Phys. Lett., 2008, 25(11): 4013-4015.
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