摘要Based on the interaction mechanism between tip and sample in the contact mode of a scanning probe acoustic microscope (SPAM), an active mass of the sample is introduced in the mass-spring model. The tip motion and frequency response of the sample vibration mode in the SPAM are calculated by the Lagrange equation with dissipation function. For the silicon tip and glass assemblage in the SPAM the frequency response is simulated and it is in agreement with the experimental result. The living myoblast cells on the glass slide are imaged at resonance frequencies of the SPAM system, which are 20 kHz, 30 kHz and 120 kHz. It is shown that good contrast of SPAM images could be obtained when the system is operated at the resonance frequencies of the system in high and low-frequency regions.
Abstract:Based on the interaction mechanism between tip and sample in the contact mode of a scanning probe acoustic microscope (SPAM), an active mass of the sample is introduced in the mass-spring model. The tip motion and frequency response of the sample vibration mode in the SPAM are calculated by the Lagrange equation with dissipation function. For the silicon tip and glass assemblage in the SPAM the frequency response is simulated and it is in agreement with the experimental result. The living myoblast cells on the glass slide are imaged at resonance frequencies of the SPAM system, which are 20 kHz, 30 kHz and 120 kHz. It is shown that good contrast of SPAM images could be obtained when the system is operated at the resonance frequencies of the system in high and low-frequency regions.
[1] Kolosov O V, Castell M R, Marsh C D and Briggs G A D 1998 Phys. Rev. Lett. 81 1046 [2] Striegler A, K\"{o}hler B and Bendjus B 2007 Proc. SPIE 6528 65281B [3] Passeri D, Rossi M, Alippi A, Bettucci A, Manno D, Serra A, Filippo E, Lucci M and Davoli I 2008 Superlattices Microstruct. 44 641 [4] Yin Q R, Yu H F, Zeng H R, Li G R and Xu Z K 2005 Mater. Sci. Eng. B 120 100 [5] Zhao K Y, Zeng H R, Song H Z, Hui S X, Li G R, Yin Q R, Shimamura K, Kannan C V, Villora E A G, Takekawa S and Kitamura K 2008 Chin. Phys. Lett. 25 3429 [6] Diebold A C 2005 Science. 310 61 [7] Shekhawat G S and Dravid V P 2005 Science. 310 89 [8] Tittmann B R, Du J, Ebert A and Piccione B 2005 Proc. SPIE 5768 11 [9] Ebert A, Tittmann B R, Du J and Scheuchenzuber W 2006 Ultrasound Med. Biol. 32 1687 [10] Banerjee S, Gayathri N, Shannigrahi S R, Dash S, Tyagi A K and Raj B 2007 J. Phys. D: Appl. Phys. 40 2539 [11] Li Y and Qian J Q 2009 Chin. Phys. Lett. 26 100703 [12] Banerjee S, Gayathri N, Dash S, Tyagi A K and Raj B 2005 Appl. Phys. Lett. 86 211913 [13] Rabe U Kopycinska M, Hirsekorn S and Arnold W 2002 Ultrasonics 40 49 [14] Burnham N A, Kulik A J, Gremaud G, Gallo P J and Oulevey F 1996 J. Vac. Sci. Technol. B 14 794 [15] Cantrell J H and Cantrell S A 2008 Phys. Rev. B 77 165409 [16] Zhao Y J, Cheng Q and Qian M L 2008 Tech. Acoust. 27 180 (in Chinese) [17] Turner J A, Hirsekorn S, Rabe U and Arnold W 1997 J. Appl. Phys. 82 966 [18] Cantrell S A, Cantrell J H and Lillehei P T 2007 J. Appl. Phys. 101 114324 [19] Johnson K L 1985 Contact Mechanics (London: Cambridge University) [20] Fischer-Cripps A C 1999 J. Mater. Sci. 34 129 [21] Kogut L and Etsion I 2002 J. Appl. Mech. 69 657 [22] Beards C F 1983 Structual Vibration Analysis (Chichester: Ellis Horwood)