A New Method for Determining Dipole Moment Orientation of Single Molecules
WANG Chen, LIU Li, WANG Gui-Ying, XU Zhi-Zhan
Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800
A New Method for Determining Dipole Moment Orientation of Single Molecules
WANG Chen;LIU Li;WANG Gui-Ying;XU Zhi-Zhan
Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800
关键词 ：
42.25.Ja ,
33.70.Ca ,
07.60.Pb
Abstract ： A new imaging method is proposed to determine the three-dimensional dipole moment orientation of single fluorophore. Far-field microscopy can provide orientational information projected in the sample plane, while total internal reflection fluorescence microscopy (TIRFM) can offer the knowledge perpendicular to the surface because longitudinal electric-field components can be generated in total internal reflection geometry. By comparing fluorescence intensities measured with far-field epi-fluorescence microscopy and TIRFM, the exact information of single-fluorescent-molecule orientation is extracted. Detailed analysis of the method is given with a numerical example.
Key words ：
42.25.Ja
33.70.Ca
07.60.Pb
出版日期: 2004-05-01
:
42.25.Ja
(Polarization)
33.70.Ca
(Oscillator and band strengths, lifetimes, transition moments, and Franck-Condon factors)
07.60.Pb
(Conventional optical microscopes)
引用本文:
WANG Chen;LIU Li;WANG Gui-Ying;XU Zhi-Zhan. A New Method for Determining Dipole Moment Orientation of Single Molecules[J]. 中国物理快报, 2004, 21(5): 843-845.
链接本文:
https://cpl.iphy.ac.cn/CN/
或
https://cpl.iphy.ac.cn/CN/Y2004/V21/I5/843
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