Detection of Fluorescence from Single Chlorophyll a Molecules Absorbed on Glass Surface
JI Dong-Mei1,2, HUANG Zheng-Xi1,2, XIA An-Dong1
1The State Key Laboratory of Molecular Reaction Dynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080
2Graduate School of Chinese Academy of Sciences, Beijing 100080
Detection of Fluorescence from Single Chlorophyll a Molecules Absorbed on Glass Surface
JI Dong-Mei1,2;HUANG Zheng-Xi1,2;XIA An-Dong1
1The State Key Laboratory of Molecular Reaction Dynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080
2Graduate School of Chinese Academy of Sciences, Beijing 100080
Abstract： We investigate the single molecule spectroscopy of chlorophyll a molecules on glass surface in N2-saturated environment. The basic photodynamic parameters of chlorophyll a molecules, such as fluorescence lifetime, survival time before photobleaching, on-time, and off-time, are reported. A four-level model is employed to describe the possible dynamics and photobleaching of chlorophyll a upon excitation. Broad distributions in fluorescence lifetimes and survival times are mainly due to the heterogeneities of both molecular conformation and local environment.
JI Dong-Mei;HUANG Zheng-Xi;XIA An-Dong. Detection of Fluorescence from Single Chlorophyll a Molecules Absorbed on Glass Surface[J]. 中国物理快报, 2005, 22(2): 317-320.
JI Dong-Mei, HUANG Zheng-Xi, XIA An-Dong. Detection of Fluorescence from Single Chlorophyll a Molecules Absorbed on Glass Surface. Chin. Phys. Lett., 2005, 22(2): 317-320.
2005, Vol. 22 
