Displacement and Force Measurements with Quadrant Photodetector in Optical Tweezers
GUO Hong-Lian1, LIU Chun-Xiang1, LI Zhao-Lin1, DUAN Jian-Fa2, HAN Xue-Hai2, CHENG Bing-Ying1, ZHANG Dao-Zhong1
1Optical Physics Laboratory, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 100080
2State Key Laboratory of Biomacromolecles, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101
Displacement and Force Measurements with Quadrant Photodetector in Optical Tweezers
1Optical Physics Laboratory, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 100080
2State Key Laboratory of Biomacromolecles, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101
Abstract： A technique of displacement and force measurements with a photodiode quadrant detector in an optical tweezers system is presented. The stiffness of optical trap is calibrated and the leukemia cell membrane tension is measured. The results show that the optical tweezers combined with the quadrant detector is a very useful tool for detecting the displacement and force with a millisecond-order response.
GUO Hong-Lian;LIU Chun-Xiang;LI Zhao-Lin;DUAN Jian-Fa;HAN Xue-Hai;CHENG Bing-Ying;ZHANG Dao-Zhong. Displacement and Force Measurements with Quadrant Photodetector in Optical Tweezers[J]. 中国物理快报, 2003, 20(6): 950-952.
GUO Hong-Lian, LIU Chun-Xiang, LI Zhao-Lin, DUAN Jian-Fa, HAN Xue-Hai, CHENG Bing-Ying, ZHANG Dao-Zhong. Displacement and Force Measurements with Quadrant Photodetector in Optical Tweezers. Chin. Phys. Lett., 2003, 20(6): 950-952.
2003, Vol. 20 
