Effects of Substrate Hydrophobicity/Hydrophilicity on Height Measurement of Individual DNA Molecules
WANG Hua-Bin 1,5, ZHOU Xing-Fei 2, AN Hong-Jie 3, GUO Yun-Chang 1, SUN Jie-Lin 4, ZHANG Yi 1, HU Jun 1,4
1Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 2018002Department of Physics, Ningbo University, Ningbo 3152113Tianjin University of Science and Technology, Tianjin 3000404Bio-X Life Science Research Center, Shanghai Jiao TongUniversity, Shanghai 2000305Graduate School of the Chinese Academy of Sciences, Beijing 100080
Effects of Substrate Hydrophobicity/Hydrophilicity on Height Measurement of Individual DNA Molecules
WANG Hua-Bin 1,5;ZHOU Xing-Fei 2;AN Hong-Jie 3, GUO Yun-Chang 1;SUN Jie-Lin 4;ZHANG Yi 1; HU Jun 1,4
1Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 2018002Department of Physics, Ningbo University, Ningbo 3152113Tianjin University of Science and Technology, Tianjin 3000404Bio-X Life Science Research Center, Shanghai Jiao TongUniversity, Shanghai 2000305Graduate School of the Chinese Academy of Sciences, Beijing 100080
摘要Effects of substrate hydrophobicity/hydrophilicity on height measurement of individual ds-DNA molecules are investigated with tapping mode atomic force microscopy (TMAFM) and vibrating mode scanning polarization force microscopy (VSPFM). The measured heights of ds-DNA on hydrophobic highly oriented pyrolytic graphite (HOPG) are remarkably less than those on hydrophilic bare mica and Ni 2+ treated mica in both TMAFM and VSPFM. By analysing the results, we propose that the hydrophobicity/hydrophilicity of substrate can greatly influence the height measurement of DNA molecules.
Abstract：Effects of substrate hydrophobicity/hydrophilicity on height measurement of individual ds-DNA molecules are investigated with tapping mode atomic force microscopy (TMAFM) and vibrating mode scanning polarization force microscopy (VSPFM). The measured heights of ds-DNA on hydrophobic highly oriented pyrolytic graphite (HOPG) are remarkably less than those on hydrophilic bare mica and Ni 2+ treated mica in both TMAFM and VSPFM. By analysing the results, we propose that the hydrophobicity/hydrophilicity of substrate can greatly influence the height measurement of DNA molecules.
WANG Hua-Bin;ZHOU Xing-Fei;AN Hong-Jie; GUO Yun-Chang;SUNJie-Lin;ZHANG Yi; HU Jun;. Effects of Substrate Hydrophobicity/Hydrophilicity on Height Measurement of Individual DNA Molecules[J]. 中国物理快报, 2007, 24(3): 644-647.
WANG Hua-Bin, ZHOU Xing-Fei, AN Hong-Jie, GUO Yun-Chang, SUNJie-Lin, ZHANG Yi, HU Jun,. Effects of Substrate Hydrophobicity/Hydrophilicity on Height Measurement of Individual DNA Molecules. Chin. Phys. Lett., 2007, 24(3): 644-647.
[1] Konig P and Richmond T J 1993 J. Mol. Biol. 233 139 [2] Morikis D and Lambris J D 2004 Trends. Immunol. 25 700 [3] Wilchek M et al 2006 Immunol. Lett. 103 27 [4] Binnig G et al 1986 Phys. Rev. Lett. 56 930 [5] Wei Z and Zhao Y P 2004 Chin. Phys. Lett. 21 616 [6] Hu J et al 1996 Langmuir 12 1697 [7] Gaczynska M et al 2004 Proc. Natl. Acad. Sci. U.S.A. 101 17952 [8] Alessandrini A and Facci P 2005 Meas. Sci. Technol. 16 R65 [9] Muller D J et al 1997 J. Struct. Biol. 119 172 [10] Kowalewski T and Holtzman DM 1999 Proc. Natl. Acad.Sci. U.S.A. 96 3688 [11] Zhang F et al 2006 Angew. Chem. Int. Ed. 45 3611 [12] Brown C L et al 2002 J. Am. Chem. Soc. 124 6846 [13] Sherratt M J et al 2004 Biophys. J. 86 3211 [14] Li X J et al 2003 J. Vac. Sci. Technol. B 21 1070 [15] Watson J D and Crick F H 1953 Nature 171 737 [16] Lyubchenko Y L et al 1993 Nucleic Acids Res. 21 1117 [17] Bustamante C et al 1997 Curr. Opin. Struct. Biol. 7 709 [18] Shaiu W L et al 1993 Nucleic Acids Res. 21 99 [19] Hu J et al 1995 Science 268 267 [20] Hu J et al 1995 Appl. Phys. Lett. 67 476 [21] Zhou X F et al 2005 Phys. Rev. E 71 062901 [22] Zhou X F et al 2005 Chem. Lett. 134 1488 [23] Zhang L J and Wang H B 2006 Chin. Phys. Lett. 23 2315 [24] Moreno-Herrero F et al 2003 Ultramicroscopy 96 167 [25] Pastre D et al 2003 Biophys. J. 85 2507 [26] Jiang X H and Lin X Q 2004 Electrochem. Commun. 6 873 [27] Vaddiraju S et al 2005 Nano. Lett. 5 1625
2007, Vol. 24 
