Humidity Effects on Imaging and Nanomanipulation of Individual DNA Molecules on HOPG Surface
ZHOU Xing-Fei1, ZHANG Yi2, WANG Hua-Bin2, NIU Dong-Xiao2, WANG Pei-Qing1, HU Jun2,3
1Department of Physics, College of Sciences, Ningbo University, Ningbo 3152112Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 2018003Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200240
Humidity Effects on Imaging and Nanomanipulation of Individual DNA Molecules on HOPG Surface
1Department of Physics, College of Sciences, Ningbo University, Ningbo 3152112Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 2018003Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200240
摘要Single DNA molecules are aligned on highly ordered pyrolytic graphite (HOPG) surface in ambient air. It is shown that environmental humidity has a remarkable influence on the measured height of DNA by atomic force microscopy (AFM), probably due to the conformation transition of DNA. We also demonstrate that DNA molecules deposited on HOPG surfacecan be `pushed' much more easily by AFM tip at high humidity than at low one.
Abstract：Single DNA molecules are aligned on highly ordered pyrolytic graphite (HOPG) surface in ambient air. It is shown that environmental humidity has a remarkable influence on the measured height of DNA by atomic force microscopy (AFM), probably due to the conformation transition of DNA. We also demonstrate that DNA molecules deposited on HOPG surfacecan be `pushed' much more easily by AFM tip at high humidity than at low one.
ZHOU Xing-Fei;ZHANG Yi;WANG Hua-Bin;NIU Dong-Xiao;WANGPei-Qing;HU Jun;. Humidity Effects on Imaging and Nanomanipulation of Individual DNA Molecules on HOPG Surface[J]. 中国物理快报, 2007, 24(9): 2692-2695.
ZHOU Xing-Fei, ZHANG Yi, WANG Hua-Bin, NIU Dong-Xiao, WANGPei-Qing, HU Jun,. Humidity Effects on Imaging and Nanomanipulation of Individual DNA Molecules on HOPG Surface. Chin. Phys. Lett., 2007, 24(9): 2692-2695.
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