1Optical Physics Laboratory, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 1000802State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094
Measurement of Binding Force between Microtubule-Associated Protein and Microtubule
1Optical Physics Laboratory, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 1000802State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094
摘要Microtubule-associated proteins (MAPs) are important proteins in cells. They can regulate the organization, dynamics and function of microtubules. We measure the binding force between microtubule and a new plant MAP, i.e. AtMAP65-1, by dual-optical tweezers. The force is obtained to be 14.6±3.5pN from the data statistics and analysis. This force measurement is helpful to understand the function and mechanism of MAPs from the mechanical point of view and lays the groundwork for future measurements of the mechanical properties of other biological macro-molecules.
Abstract：Microtubule-associated proteins (MAPs) are important proteins in cells. They can regulate the organization, dynamics and function of microtubules. We measure the binding force between microtubule and a new plant MAP, i.e. AtMAP65-1, by dual-optical tweezers. The force is obtained to be 14.6±3.5pN from the data statistics and analysis. This force measurement is helpful to understand the function and mechanism of MAPs from the mechanical point of view and lays the groundwork for future measurements of the mechanical properties of other biological macro-molecules.
XU Chun-Hua;GUO Hong-Lian;QU E;LI Zhao-Lin;YUAN Ming;CHENGBing-Ying;ZHANG Dao-Zhong. Measurement of Binding Force between Microtubule-Associated Protein and Microtubule[J]. 中国物理快报, 2007, 24(9): 2714-2416.
XU Chun-Hua, GUO Hong-Lian, QU E, LI Zhao-Lin, YUAN Ming, CHENGBing-Ying, ZHANG Dao-Zhong. Measurement of Binding Force between Microtubule-Associated Protein and Microtubule. Chin. Phys. Lett., 2007, 24(9): 2714-2416.
[1] Dye R B, Fink S P and Williams R C Jr 1993 J. Biol. Chem. 268 6847 [2] Smertenko A P et al 2004 Plant Cell 16 2035 [3] Arai Y et al 1999 Nature 399 446 [4] Felgner H, Frank R and Schliwa M 1996 J. Cell Sci. 109509 [5] Kerssemakers J W J et al 2003 Appl. Phys. Lett. 83 4441 [6] Finer J T, Simmons R M and Spudich J A 1994 Nature 368 113 [7] Kienberger F et al 2005 J. Mol. Boil. 347 597 [8] Gao H, Zhang X X and Chang W B 2005 Front. Biosci. 101539 [9] Avci R et al 2004 Langmuir 20 11053 [10] Keating T J et al 1997 Proc. Natl. Acad. Sci. 94 5078 [11] Liu C X et al 2005 Chin. Phys Lett. 22 1278 [12] Mao T et al 2005 Plant Physiol. 138 654 [13]Guo H L et al 2002 Sci. Chin. 45 919 [14]Guo H L et al 2003 Chin. Phys. Lett. 20 950 [15]Liu C X et al 2005 Acta Phys. Sin. 54 1162 (in Chinese) [16] Kis A et al 2002 Phys. Rev. Lett. 89 248101 [17] Tsuda Y et al 1996 Proc. Natl. Acad. Sci. 93 12937 [18] Moy V T, Florin E L and Gaub H E 1994 Science 266 257
2007, Vol. 24 
