Applications of Oblique-Incidence Reflectivity Difference Method in Primary Study of Protein Biomolecules
ZHANG Hong-Yan1, LIANG Ru-Qiang2, JIN Kui-Juan1, Lü Hui-Bin1, ZHU Xiang-Dong3, ZHOU Yue-Liang1, RUAN Kang-Cheng2, YANG Guo-Zhen1
1Laboratory of Optical Physics, Institute of Physics and Center for Condensed Mater Physics, Chinese Academy of Sciences, Beijing 100080
2Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031
3Department of Physics, NSF Center for Biophotonics Science and Technology (CBST), University of California at Davis, California 95817, USA
Applications of Oblique-Incidence Reflectivity Difference Method in Primary Study of Protein Biomolecules
1Laboratory of Optical Physics, Institute of Physics and Center for Condensed Mater Physics, Chinese Academy of Sciences, Beijing 100080
2Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031
3Department of Physics, NSF Center for Biophotonics Science and Technology (CBST), University of California at Davis, California 95817, USA
Abstract： Oblique-incidence reflectivity difference (OI-RD) analysis is applied to detect the immunoglobulin-G and cytochrome biomolecules on standard glass substrates without fluorescence labelling. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential applications in detection of biochemical processes.
ZHANG Hong-Yan;LIANG Ru-Qiang;JIN Kui-Juan;Lü Hui-Bin;ZHU Xiang-Dong;ZHOU Yue-Liang;RUAN Kang-Cheng;YANG Guo-Zhen. Applications of Oblique-Incidence Reflectivity Difference Method in Primary Study of Protein Biomolecules[J]. 中国物理快报, 2006, 23(4): 1032-1033.
ZHANG Hong-Yan, LIANG Ru-Qiang, JIN Kui-Juan, Lü Hui-Bin, ZHU Xiang-Dong, ZHOU Yue-Liang, RUAN Kang-Cheng, YANG Guo-Zhen. Applications of Oblique-Incidence Reflectivity Difference Method in Primary Study of Protein Biomolecules. Chin. Phys. Lett., 2006, 23(4): 1032-1033.
2006, Vol. 23 
