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Examination of Validity of Paraxial Approximation in Second Harmonic Generation Microscopy under Low Numerical Aperture |
| WANG Xiang-Hui, LIN Lie, CHANG Sheng-Jiang, ZHANG Hao, HAO Shu-Juan |
| Institute of Modern Optics, Key Laboratory of Opto-electronic Information Science and Technology (Ministry of Education), Nankai University, Tianjin 300071 |
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| Cite this article: |
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WANG Xiang-Hui, LIN Lie, CHANG Sheng-Jiang et al 2008 Chin. Phys. Lett. 25 2884-2887 |
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Abstract By using a vectorial approach, the validity of paraxial approximation in second harmonic generation (SHG) microscopy under low numerical aperture (NA) is examined when the sample is a collagen fibril. Due to the larger value of dzzx and tensorial nature of SHG, the component Ez of the focused field may have strong effect on the radiation pattern of SHG. Numerical results indicate that when the value of NA exceeds 0.3, the effect of Ez can not be neglected, which results in the invalidation of paraxial approximation in SHG microscopy despite the fact that SHG microscopy is still under low NA focusing.
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| Keywords:
42.65.Ky
87.64.Mn
87.50.Wf
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Received: 14 April 2008
Published: 25 July 2008
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| PACS: |
42.65.Ky
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(Frequency conversion; harmonic generation, including higher-order harmonic generation)
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87.64.mn
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(Multiphoton)
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87.50.wf
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(Biophysical mechanisms of interaction)
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