Chin. Phys. Lett.  2007, Vol. 24 Issue (9): 2714-2416    DOI:
Original Articles |
Measurement of Binding Force between Microtubule-Associated Protein and Microtubule
XU Chun-Hua1;GUO Hong-Lian1;QU E1;LI Zhao-Lin1;YUAN Ming2;CHENG
Bing-Ying1;ZHANG Dao-Zhong1
1Optical Physics Laboratory, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 1000802State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094
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XU Chun-Hua, GUO Hong-Lian, QU E et al  2007 Chin. Phys. Lett. 24 2714-2416
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Abstract Microtubule-associated proteins (MAPs) are important proteins in cells. They can regulate the organization, dynamics and function of microtubules. We measure the binding force between microtubule and a new plant MAP, i.e. AtMAP65-1, by dual-optical tweezers. The force is obtained to be 14.6±3.5pN from the data statistics and analysis. This force measurement is helpful to understand the function and mechanism of MAPs from the mechanical point of view and lays the groundwork for future measurements of the mechanical properties of other biological macro-molecules.
Keywords: 87.15.La      87.14.Ee      87.80.Cc     
Received: 10 January 2007      Published: 16 August 2007
PACS:  87.15.La (Mechanical properties)  
  87.14.Ee  
  87.80.Cc (Optical trapping)  
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https://cpl.iphy.ac.cn/       OR      https://cpl.iphy.ac.cn/Y2007/V24/I9/02714
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XU Chun-Hua
GUO Hong-Lian
QU E
LI Zhao-Lin
YUAN Ming
CHENGBing-Ying
ZHANG Dao-Zhong
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