| Original Articles |
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Mechanical Properties of Breast Cancer Cell Membrane Studied with Optical Tweezers |
| GUO Hong-Lian1;LIU Chun-Xiang1;DUAN Jian-Fa2;Jiang Yu-Qiang1;HAN Xue-Hai2;LI Zhao-Lin1;CHENG Bing-Ying1;ZHANG Dao-Zhong1 |
| 1Optical Physics Laboratory, Institute of Physics, Chinese Academy of Sciences, Beijing 100080
2State Key Laboratory of Biomacromolecles, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 |
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| Cite this article: |
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GUO Hong-Lian, LIU Chun-Xiang, DUAN Jian-Fa et al 2004 Chin. Phys. Lett. 21 2543-2546 |
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Abstract Membrane tethers are extracted from breast cancer cells using a force generated by an optical trap. It is experimentally obtained that the radius of tether is about 0.1μm and the static tether force is about
8.5 pN. Calculations based on the experimental measurements give a bending modulus for the tether of 1.35×10-19Nm and a surface membrane tension of 6.76×10-6N/m in the breast cancer
cell. The treatment with cytochalasin D results in the decreasing bending modulus and decreasing apparent surface tension. When the membrane protein caveolin is over-expressed, similar cases occur in bending modulus and apparent surface tension. In addition, the viscous resistance coefficient of the membrane is calculated to be 1.15pN.s/μm according to the dynamic tether forces obtained under different pulling velocities.
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81.70.Fy
87.15.La
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Published: 01 December 2004
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| PACS: |
81.70.Fy
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(Nondestructive testing: optical methods)
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87.15.La
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(Mechanical properties)
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