| GENERAL |
|
|
|
|
Label-Free and Real-Time Detection of Antigen-Antibody Capture Processes Using the Oblique-Incidence Reflectivity Difference Technique |
| HE Li-Ping 1,2, DAI Jun1, SUN Yue1,2, WANG Jing-Yi1, LÜ Hui-Bin1**, WANG Shu-Fang2**, JIN Kui-Juan1, ZHOU Yue-Liang1, YANG Guo-Zhen1 |
1Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190
2College of Physics Science and Technology, Hebei University, Baoding 071002 |
|
| Cite this article: |
|
|
|
|
|
|
Abstract We successfully label-free and real-time detect the capture processes of human immunoglobulin G (IgG)/goat anti-human IgG and mouse IgG/goat anti-mouse IgG antigen-antibody pairs with different concentrations using the oblique-incidence reflectivity difference (OIRD) method, and obtain the interaction kinetics curves and the interaction times. The experimental results prove that the OIRD method is a promising technique for label-free and real-time detection of the biomolecular interaction processes and achieving the quantitative information of interaction kinetics.
|
|
|
Received: 13 February 2012
Published: 29 July 2012
|
|
| PACS: |
07.60.Fs
|
(Polarimeters and ellipsometers)
|
| |
81.70.Fy
|
(Nondestructive testing: optical methods)
|
| |
87.80.Dj
|
(Spectroscopies)
|
| |
83.85.Ei
|
(Optical methods; rheo-optics)
|
|
|
|
|
|
[1] Zhu H, Bilgin M, Bangham R, Hall D, Casamayor A, Bertone P, Lan N, Jansen R, Bidlingmaier S, Houfek T, itchell T, Miller P, Dean R A, Gerstein M and Snyder M 2001 Science 293 2101
[2] Espina V, Woodhouse E C, Wulfkuhle J, Asmussen H D, Petricoin E F and Liotta L A 2004 J. Immunol. Methods 290 121
[3] Yu X B, Xu D K and Cheng Q 2006 Proteomics 6 5493
[4] Ray S, Mehta G and Srivastava S 2010 Proteomics 10 731
[5] Han M, Gao X, Su J Z and Nie S 2001 Nature Biotechnol. 19 631
[6] Gao X, Cui Y, Levenson R M, Chung L W K and Nie S M 2004 Nature Biotechnol. 22 969
[7] Li P Y, Lin B, Gerstenmaier J and Cunningham B T 2004 Sens. Actuat. B 99 6
[8] Zhu X D, Landry J P, Sun Y S, Gregg J P, Lam K S and Guo X W 2007 Appl. Opt. 46 1890
[9] Fei Y Y, Landry J P, Sun Y S, Zhu X D, Luo J T, Wang X B and Lam K S 2008 Rev. Sci. Instrum. 79 013708
[10] Wang X, Yuan K, Lu H, Wen J, Lu H B, Jin K J, Zhou Y L, Yang G Z, Li W and Ruan K C 2010 J. Appl. Phys. 107 063109
[11] Wang X, Lu H, Wen J, Yuan K, Lu H B, Jin K J, Zhou Y L and Yang G Z 2010 Chin. Phys. Lett. 27 107801
[12] Lu H, Wen J, Wang X, Yuan K, Lu H B, Zhou Y L Jin K J, Ruan K C and Yang G Z 2010 J. Opt. 12 095301
[13] Yuan K, Wang X, Lu H, Wen J, Lu H B, Zhou Y L, Jin K J, Yang G Z, Li W and Ruan K C 2010 Sci. Chin. Mech. Astron. 53 1434
[14] Lu H, Wen J, Wang X, Yuan K, Lu H B, Zhou Y L, Jin K J, Yang G Z, Li W and Ruan K C 2010 Sci. Chin. Mech. Astron. 53 1230
[15] Wen J, Lu H, Wang X, Yuan K, Lu H B, Zhou Y L, Jin K J, Yang G Z, Li W and Ruan K C 2010 Sci. Chin. Mech. Astron. 53 306
[16] Liang R Q, Tan C Y and Ruan K C 2004 J. Immunol. Methods 285 157 |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
